TABLE 4.
HA secreted into the medium
The values shown are from preadipocytes from orbital samples in response to expression of activating mutant TSHR (L629F); following 24 h of treatment with 10−3 m db-cAMP (db-cAMP); following treatment for 9 days with 5 milliunits/ml TSH, or a monoclonal thyroid stimulating antibody (TSAB). NS, not significant. The values are reported as ng/104 cells (means ± S.E.).
| Control | Stimulated | p value | |
|---|---|---|---|
| Normal and GO combined | |||
| L629F TSHR, n = 6 | 425 ± 100 | 528 ± 107 | p < 0.05 (Wilcoxon) |
| db-cAMP, n = 4 | 113 ± 16 | 165 ± 26 | p < 0.08 (Student's) |
| Normal and GO separately | |||
| TSAB, normal n = 3 | 318 ± 103 | 377 ± 123 | p < 0.05 (Student's) |
| TSH, normal n = 3 | 318 ± 103 | 395 ± 135 | p < 0.06 (Student's) |
| TSAB, GO n = 6 | 417 ± 93 | 317 ± 106 | NS (Student's)a |
| TSH, GO n = 6 | 417 ± 93 | 317 ± 110 | NS (Student's)a |
a Response of GO preadipocytes to both ligands significantly lower than that of normal preadipocytes (p < 0.05).