FIGURE 1.
Oct-1 binds to Cdx-2 promoter in vivo. A, comparison of the nucleotide sequences of the mouse Cdx-2 gene proximal promoter region with that of its homologues in hamsters (Cdx-3), humans (CDX2), and chickens (Cdx-C). TATA, TATA box; DBS, downstream binding site (36). B, schematic representation of the hamster Cdx-2 gene 5′-flanking region (GenBankTM accession number EF530070), positions of Cdx-2pOCT, and two primers utilized in the ChIP assay. C, Oct-1, RNA polymerase II (RNA pol II), and p300 antibody-precipitated Cdx-2 promoter containing genomic DNA (but not the control DNA) in InR1-G9 cells detected by ChIP. D, forskolin/IBMX treatment (F/I, 10 μm each) reduced in in vivo binding of Oct-1 to hamster Cdx-2 promoter, detected by quantitative ChIP. V, vehicle for forskolin/IBMX. E, forskolin/IBMX treatment (F/I, 10 μm each) enhanced binding of RNA pol II to Cdx-2 promoter, detected by quantitative ChIP.