FIGURE 3.

Oct-1 knockdown stimulates Cdx-2 and gcg expression. A, Oct-1 siRNA3 significantly repressed Oct-1 expression. InR1-G9 cells were transfected with 50 nm of control (scrambled) or an indicated Oct-1 siRNA for 36 h. Cells were harvested, and whole cell lysates were assessed for Oct-1 expression by Western blotting. B, reduced Oct-1 expression in InR1-G9 cells was accompanied by increased Cdx-2 protein expression, detected by Western blotting. C, Oct-1 knockdown led to increased Cdx-2 and gcg mRNA expression. InR1-G9 cells were transfected with 50 nm control or Oct-1 siRNA3. Total RNA was isolated and assessed for Cdx-2 and gcg mRNA expression by real time RT-PCR. Values are mean ± S.E. (n > or = 3). *, p < 0.05.