FIGURE 7.

Forskolin/IBMX treatment reduces nuclear Oct-1 content in primary pancreatic islet cells. A, Wistar rat pancreatic islets were treated with or without forskolin/IBMX (F/I) (10 μm each) for 2 h. V, vehicle. Nuclear proteins were extracted, and Oct-1 contents were assessed by Western blotting (left panel) and quantified by densitometric analysis (right panel) (n = 3). B and C, real time RT-PCR shows that forskolin/IBMX treatment enhanced proinsulin I (Ins I, B) and proglucagon (Gcg, C) mRNA expression. Values are mean ± S.E. (n = 4). *, p < 0.05.