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Forskolin/IBMX treatment or Epac activation stimulates nuclear exclusion of Myc-tagged Oct-1. InR1-G9 cells transfected with Oct-1-Myc were treated with vehicle (V), or forskolin/IBMX (10 μm each, F/I), or 8-pMeOPT-2′-O-Me-cAMP (ESCA, 20 μm), or ESCA plus H89, or ESCA plus PD98059 for 1 h. The intracellular distribution of Oct-1-Myc was examined by immunostaining. 4′,6-Diamidino-2-phenylindole (DAPI), nuclear staining; DIC, differential interference contrast.
