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. 2009 Jul 27;284(39):26482–26492. doi: 10.1074/jbc.M109.009340

FIGURE 4.

FIGURE 4.

Comparison of Pro and P5C content in p5cdh and WT Arabidopsis plants during drought stress and recovery and correlation with the transcript levels of genes involved in Pro biosynthesis and oxidation. Three-week-old WT and p5cdh plants were dehydrated for 72 h and thereafter recovered by adding the water volume lost by evaporation. A, RNA blot analysis of total RNA (10 μg/lane) probed with P5CDH, ProDH, P5CS, and P5CR cDNA fragments. B and C, leaf Pro and P5C content during drought and recovery. The inset in B displays Pro levels normalized to dry weight, showing that during 24 h of water absorption in the recovery period, no loss of Pro is observed in the p5cdh plants. The standard deviation represents three leaf samples, each pooled from 15–20 plants.