FIGURE 7.

Exogenous Pro application induces mitochondrial ROS generation in WT and p5cdh roots under dark conditions. Confocal root tip images of 5-day-old WT (A, C, E, and G) and p5cdh (B, D, F, H, and I) seedlings exposed to the following treatments for 24 h in the dark: A and B, control, no treatment; C and D, 100 mm Pro; E and F, 100 μm PQ; G–I, 100 mm Pro + 100 μm PQ. Left column, Numarski images. MitoSox fluorescence images are in green pseudo-color (second from the left). MitoTracker Deep-Red images are in magenta pseudo-color (third from the left), and the merged fluorescence is in the right column. Nuclear localization of MitoSox (I) is shown by costaining with 4′,6-diamidino-2-phenylindole (blue). Bar, 25 mm.