FIGURE 3.

Susceptibility of the LEC-8-deficient mutant lec-8(tm1477) to t-Cry5B toxicity. A, genomic structure of the lec-8 locus. The position of the deletion in the mutant allele (tm1477) is indicated (horizontal line). A 3′-untranslated region (3′-UTR) is indicated as a gray box. B, toxicity assay in solution. Young adult N2 and lec-8(tm1477) worms were picked individually and transferred into the wells of 96-well microtiter plates containing various concentrations of purified t-Cry5B as described under “Experimental Procedures.” To score for susceptibility, animals were examined after 18 and 24 h using a dissecting microscope. The percentage of dead worms at each time point was divided by the number of worms at the start of the experiment. Black bars represent the proportion of dead worms. Shaded bars are the proportion of worms that exhibited toxic effects (abnormal movement and morphology, culminating in death within several hours). Assay was performed at 20 °C with ≥20 worms per condition. Data shown represent typical results from three experiments.