FIGURE 2.

Expression of P1c in spinal cord and sciatic nerve. A–D, immunohistochemistry (diaminobenzidine staining) of spinal cord sections. Rectangles marked in A and C are shown magnified in C and D, respectively. Note the prominent staining of WT gray matter in A and the positive staining of motor neurons and smaller interneurons in C and of the cell body and processes of a motor neuron in D. E and F, IFM of sciatic nerve cross-sections from WT (E) and P1c^−/− mice (F) reveals strong A1c signals in Schwann cells (arrowheads) and relatively weak staining of axons (arrow). G and H, primary Schwann cells from WT (G) and P1c^−/− mice (H). The weak nuclear staining in F and H is nonspecific. Scale bars, 200 μm (A and B), 50 μm (C), 20 μm (G and H), and 10 μm (D–F).