FIGURE 5.

Ultrastructural analysis of L5 ventral roots of P1c^−/− mice and their WT littermates. A, EM of cross-sectioned L5 ventral root motor axons from WT and P1c^−/− mice. Scale bar, 200 nm. B and C, nearest neighbor analyses of NF (B) and MT (C) spacing in WT and P1c^−/− mice. Preparations of L5 ventral roots from three pairs of WT and P1c^−/− littermates were analyzed (in total 41 and 32 axons, respectively). D and E, NF (D) and MT (E) densities in WT and P1c^−/− mice. A template of hexagons was applied over each electron micrograph analyzed, and the number of NFs and MTs was counted in all hexagons (n = 1180, WT; n = 1380, P1c^−/−).