FIGURE 2.

Quantification of γ in TF₁F_o. E. coli membrane preparations containing wild-type TF₁F_o (lanes 1–5), TF₁F_oΔγ (lane 7), and no TF₁F_o (pUC118/DK8; lane 8) were run on an SDS gel and developed by Western blotting using an antibody against the globular portion of γ. The amount of wild-type TF₁F_o in the membranes loaded into lanes 1–5 was as follows: lane 1, 40 ng; lane 2, 100 ng; lane 3, 200 ng; lane 4, 400 ng; lane 5, 1 μg. Lane 6 was empty. Lane 7 contained membranes with 1 μg of TF₁F_oΔγ. Lane 8 contained the same amount of total membrane protein as lane 7 but without any TF₁F_o. The nature of the two faint bands below γ that are visible at the highest concentration of wild-type TF₁F_o is not known.