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Binding of wild type and mutated HCR/T domains to ganglioside GD3. Immobilized ganglioside GD3 (250 ng/well) was incubated with the indicated HCR domains and assayed for HCR binding as described previously (15). The data were plotted using GraphPad Prism version 5 (GraphPad Software Inc.). ■, HCR/T wild type; ▾, HCR/T (R+, W−); ▴, HCR/T (R−, W+); *, HCR/T (R−, W−).
