FIGURE 5.

Ganglioside loading of PC12 cells enhances the uptake of TeNT. PC12 cells were loaded with the indicated ganglioside. a, cells were washed and incubated with 100 nm HCR/T and 10 μg/ml of Alexa Fluor 488-Transferrin (Tfn) for 30 min at 37 °C. HCR/T was visualized by immunofluorescence using a mouse α-FLAG antibody followed by Alexa-coupled secondary IgG. b, cells were incubated with 30 nm TeNT for an additional 48 h. VAMP2 cleavage was visualized by Western blotting, using a mouse α-Syb2 antibody, which recognizes only full-length VAMP2. BSA, bovine serum albumin.