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. 2009 Jul 27;284(39):26578–26590. doi: 10.1074/jbc.M109.011775

FIGURE 2.

FIGURE 2.

PEPCK-M but not PEPCK-C is present in INS-1 832/13 cells, rat islets, and mouse islets. A and B, PEPCK-M mRNA expression in comparison to PEPCK-C mRNA expression by quantitative PCR in INS-1 832/13 cells (A) and rat islets (B). C, Western blots using an antibody specific for PEPCK-C and a peptide antibody specific for PEPCK-M in INS-1 832/13 cells, rat, and mouse islets. D, PEPCK activity in whole cells as well as isolated mitochondria of INS-1 832/13 cells, rat islets, and hepatocytes using a traditional method measuring NADH oxidation. E, PEPCK activity in the mitochondrial fraction of INS-1 832/13 cells and rat islets compared with glutamate dehydrogenase (GDH) activity. Error bars are S.E. and significance by t test; ***, p < 0.001.