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. 2009 Jul 28;284(39):26591–26602. doi: 10.1074/jbc.M109.022491

TABLE 2.

Sequence of CY 5.5-labeled oligonucleotides used for EMSA (sense sequence)

CY 5.5-labeled forward sense oligonucleotides were annealed with the respective antisense oligonucleotides. Bold sequences indicate the putative AP-1 binding site within the promoter region or the intronic region of human PUMA gene; the underlined sequences indicate the altered nucleotides.

Oligonucleotide name Oligonucleotide sequence (5′ → 3′)
Promoter AP-1 CACTGGAAGGTGAGTCACTCTGGTGAGGCG
Intronic AP-1 aagctgagaaattgactcactagcgatggg
Promoter AP-1 mutant CACTGGAAGGAACTGCACTCTGGTGAGGCG
Intronic AP-1 mutant aagctgagaaattgcagttctagcgatggg