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Primer sets used for amplification of chromatin immunoprecipitation DNA
TSS, transcription start site; F, forward; R, reverse.
| Promoter region | Location from TSS | Primer sequence (5′ → 3′) | Product size |
|---|---|---|---|
| bp | |||
| PUMA promoter #1 AP-1 | −121 | F: AGATTACCTGCATCTCTTGG | 229 |
| +108 | R: CCCTGCTCTGGTTTGGTGAGT | ||
| PUMA promoter #2, nonspecific | −8372 | F: GCTCACCAAGTCTGGAGCT | 125 |
| −8247 | R: CGAGAGACCGTAACGTGCAT | ||
| JUN promoter | −140 | F: tgtaggagggcagcggagcattacc | 197 |
| AP-1 | +57 | R: gcccgagctcaacacttatctgctaccag |
