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Western analysis of functional cell extracts. A, hLigI, MSH2, MSH6, FEN-1, PCNA, and actin proteins levels are shown. B, phosphorylation status of hLigI of the same extracts shown in A with and without calf intestinal alkaline phosphatase (CIP) treatment. Protein amount was equally loaded in all cases. Blots are representative examples of three independent analyses.
