TABLE 1.
Conditions | Rate of degradation | p value | No. imaged dendrites |
---|---|---|---|
% Fluorescence decay/2 min | |||
Controla | 2.43 ± 0.50 | 21 | |
MG132 | 0.58 ± 0.39 | 0.009 | 16 |
Vinyl sulfone | 0.55 ± 0.29 | 0.025 | 9 |
Epoxomicin | 0.87 ± 0.18 | 0.035 | 11 |
Calcium-free | 0.67 ± 0.19 | 0.012 | 13 |
APV | 1.34 ± 0.27 | 0.115 | 13 |
Nimodipine | 1.70 ± 0.05 | 0.418 | 7 |
CNQX | 2.14 ± 0.28 | 0.704 | 10 |
BIC, t 10 min | 5.67 ± 0.51 | <0.001 | 27 |
BIC, t 20 min | 4.06 ± 0.37 | 0.01 | 27 |
BIC, t 60 min | 1.65 ± 0.07 | 0.47 | 5 |
AMPA | 5.90 ± 0.34 | <0.001 | 11 |
TTX | 1.13 ± 0.10 | 0.02 | 19 |
KN-93 | 1.39 ± 0.12 | 0.076 | 16 |
paGFPb | 0.25 ± 0.25 | 0.003 | 13 |
BICa | 4.06 ± 0.37 | 27 | |
MG132 + BIC | 1.54 ± 0.31 | 0.001 | 8 |
Calpeptin + BIC | 3.92 ± 0.40 | 0.814 | 15 |
paGFPb + BIC | 1.27 ± 0.12 | <0.001 | 12 |
APV + BIC | 1.90 ± 0.26 | <0.001 | 14 |
Nimodipine + BIC | 1.67 ± 0.30 | 0.001 | 10 |
CNQX + BIC | 1.25 ± 0.16 | 0.003 | 5 |
KN-93 + BIC | 1.58 ± 0.11 | <0.001 | 17 |
a Indicates reference category.
b paGFP, no-degron control reporter.