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. 2009 Jul 31;284(39):26685–26694. doi: 10.1074/jbc.M109.013342

FIGURE 2.

FIGURE 2.

Distribution patterns of snoRNP proteins and other nuclear proteins in NS knockdown cells. A, double immunofluorescence staining of HeLa cells transfected with NS siRNAs or control siRNA. Antibodies against fibrillarin and Nop56 were used in the left set of the panels. Different cells were double-stained with antibodies against fibrillarin and Nop58 in the right set. Bar, 5 μm in A, C, and D. B, Western blotting of HeLa whole cell extracts 5 days after the second transfection of siRNAs. C, immunostaining of HeLa cells with anti-dyskerin antibody 5 days after the second transfection of siRNAs. D, HeLa cells transfected with NS siRNAs were double immunofluorescence stained with antibodies against fibrillarin and UBF. E, Western blotting of proteins prepared from HeLa cells transfected with B23 siRNA or control siRNA. F, HeLa cells transfected with B23 siRNA or control siRNA were double immunofluorescence stained with antibodies against B23 and dyskerin. Bar, 10 μm in F and G. G, HeLa cells transfected with NS siRNA-1 or control siRNA were stained with the anti-coilin antibody and Topro 3. Arrowheads indicate Cajal bodies.