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. 2009 Jul 31;284(39):26685–26694. doi: 10.1074/jbc.M109.013342

FIGURE 6.

FIGURE 6.

Disruption of the telomerase complex by NS knockdown. A, Western blotting of fractions obtained from Superdex 200 gel filtration. Anti-TERT antibody was used. B, Western blotting of TERT in HeLa cells transfected with siRNAs. C and D, Western blotting of co-immunoprecipitated proteins with anti-TERT antibody (C) and those with anti-dyskerin antibody (D) from HeLa cell extracts after siRNA transfection. E, relative total amount of TR in HeLa cells transfected with NS siRNAs. The amount in the cells transfected with control siRNA was defined as 100%. F, relative amount of TR co-immunoprecipitated with anti-TERT antibody from HeLa cells transfected with NS siRNA-1. The value obtained with control siRNA was defined as 100%. The differences of the mean values between control and NS siRNAs are statistically significant in F and G (asterisk, p < 0.05). G, relative telomerase activity, measured as TPG values, in HeLa cells transfected with NS siRNAs in comparison to that in control cells.