TABLE 1.
Primer sequences used for qRT-PCR
| Gene namea | Primer sequence (5′–3′) |
|---|---|
| GAPDH F | TGCACCACCAACTGCTTAG |
| GAPDH R | GATGCAGGGATGATGTTC |
| TR F | TCTAACCCTAACTGAGAAGGGCGT |
| TR R | TGCTCTAGAATGAACGGTGGAAGG |
| U3 F | TTCTCTGAACGTGTAGAGCACCGA |
| U3 R | GATCATCAATGGCTGACGGCAGTT |
| U8 F | CGTCAGGTGGGATAATCCTT |
| U8 R | GGGTGTTGCAAGTCCTGATT |
| U13 F | CTTTTGTAGTTCATGAGCGTGAT |
| U13 R | GGTCAGACGGGTAATGTGC |
| U15A F | ATGTTCTCTTTGCCCAGGTG |
| U15A R | CCT*TCTCAGACAAATGCCTCT |
| U16 F | GCAATGATGTCGTAATTTGCGTCT |
| U16 R | CGTCAACCTTCTGTACCAGCTTAC |
| U19 F | ATGTGGTGCCTGTGATGGTGTTAC |
| U19 R | ACACTGCCCAAAGGTACTCAGCTA |
| U23 F | GTCTTCTCATTGAGCTCCTTTCTGTC |
| U23 R | GCTAGTTCTGTGAATTCTCTCTTCTTCTCG |
| U67 F | TCAGTGCCTCTCAGGAAAGTAGCA |
| U67 R | GGCAGAGGAAATAAGCATCCCAGT |
| U70 F | AAGCCGACTGAGTTCCTTTCCTCA |
| U70 R | ACAACCAACAGGCTGCGTACACTA |
| U71A F | GCCTGTGCCTAGGTCATTGATAGT |
| U71A R | AAAGCTTCAGGGTTCGGATGGGAT |
a F and R indicate forward and reverse primers, respectively.