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. 2009 Jul 31;284(39):26803–26815. doi: 10.1074/jbc.M109.028381

FIGURE 1.

FIGURE 1.

Expression of MKP-1 and phospho-p38 in BEAS-2B and A549 cells. A, BEAS-2B and A549 cells were either not stimulated or stimulated with dexamethasone (1 μm), TNFα (10 ng/ml), or a combination of both. Cells were harvested for protein after 0.25, 0.5, 1, 2, 6, or 18 h, and cell lysates were subject to Western blot analysis for MKP-1 and GAPDH. B, BEAS-2B and A549 cells were treated with various concentrations of dexamethasone as indicated. After 1 h (BEAS-2B) or 6 h (A549), cells were harvested for Western blot analysis of MKP-1 and GAPDH. Following densitometric analysis, data (n = 3–4), normalized to GAPDH were expressed as a percentage of maximum concentration of dexamethasone and plotted as means ± S.E. C, cells treated as in A were harvested for Western blot analysis of phospho-p38 MAPK (P-p38) and GAPDH. In all cases, blots representative of 3–4 such experiments are shown.