FIGURE 4.

Galectin-1 induces increased Syk phosphorylation in human MDDCs. Western blots showing pSyk and total Syk protein in MDDC lysate (30 μg/lane) following treatment with galectin-1 (20 μm) (A) or LPS (250 ng/ml) (C) for the indicated times or 1 μg of mouse anti-CD43 monoclonal antibody or anti-CD45 monoclonal antibody (E) for 1 h. Where indicated, anti-mouse IgG was added during the last 30 min of incubation to super-cross-link the mouse monoclonal antibodies. Corresponding densitometric quantification of the pSyk/total Syk ratios is shown in B, D, and F, respectively. Galectin-1 was added to cells in the presence of polymyxin B (10 μg/ml) to eliminate any contaminating endotoxin effects.