FIGURE 1.
Insertional deletion of Slc4a11. A, restriction map of the retroviral gene trap vector, VICTR48. B, structural arrangement of 129 Sv/Evbrd mouse gene Slc4a11; diagram of the mutated Slc4a11 allele after insertion. C, immunoblot analysis of protein samples prepared from HEK293 cells transfected with wild-type NaBC1, cochlea, and cornea of wild-type (Slc4a11+/+), and knock-out (Slc4a11−/−) mice using the C-terminal antibody to CL-NaBC1. D, HEK293 cells transfected with wild-type NaBC1, showing NaBC1 immunoreactivity. E, mock-transfected HEK293 cells showing lack of NaBC1 staining; F, Nomarski image of the same field in E. G, HEK293 cells transfected with wild-type NaBC1 were not stained when the Cl-NaBC1 antibody was preincubated with the immunizing peptide. H, Nomarski image of the same field in G. LTR, long terminal repeat.