FIGURE 11.

A, corneal endothelium analysis in Slc4a11^+/+ mice; B, corneal endothelium analysis in Slc4a11^−/− mice. Corneal endothelium was stained with alizarin red to identify cell borders. No gross abnormalities were observed in endothelium from either group. Scale bar, 100 μm. Images of alizain red-stained central corneas were used to calculate central endothelial cell densities (C) and mean cell area (D). No significant differences in cell density or area were observed between Slc4a11^+/+ and Slc4a11^−/− mouse corneas.