FIGURE 13.

NaBC1 immunofluorescence in Slc4a11^+/+ and Slc4a11^−/− mouse cornea. A, NaBC1 immunofluorescence was present in layer 5 (endothelium), and less prominent but consistently observed immunofluorescence was seen in layer 1 epithelium. Much less evident was NaBC1 in the Bowman's (anterior membrane) layer 2. No staining was seen in layers 3 and 4. B, all cell nuclei stained with DAPI. C, merged image from A and B. D, NaBC1 immunofluorescence was not detected in any corneal layers of the Slc4a11^−/− mice. E, cell nuclei stained with DAPI. F, merged image from D and E. Bar in A and D, 20 μm.