FIGURE 4.

NaBC1 immunofluorescence in Slc4a11^+/+ and Slc4a11^−/− mice. A, NaBC1 immunofluorescence in the spiral ligament (SL) of the cochlea from Slc4a11^+/+ mice. A uniform NaBC1 pattern was observed from the base to apical portion of the cochlea. B, higher magnification view shows specific NaBC1 immunofluorescence in the spiral ligament and the spiral limbus (L). The organ of Corti (OC) is devoid of any immunofluorescence signal. C, NaBC1 immunofluorescence was not detected in the Slc4a11^−/− mouse cochlea. D, high magnification view to corroborate the lack of NaBC1 immunofluorescence. Bar, 250 μm (A and C) and 25 μm (B and D).