FIGURE 8.

Examples of VsEP waveforms for a typical Slc4a11^+/+ mouse (left) and a representative Slc4a11^−/− mouse (right). Stimuli were calibrated in jerk levels (change in acceleration over time, da/dt) and were defined in terms of db reference 1.0 g/ms, where 0 db produced a linear acceleration pulse with jerk amplitude of 1 g/ms (g = 9.8 m/s²). Stimulus amplitudes ranged from +6 db to −18 db. At +6 db, VsEPs were also collected with a forward masker (+6 M group), as described under “Experimental Procedures.” Each stimulus level shows two response waveforms to demonstrate response replication. As stimulus intensity is reduced, peak-to-peak amplitudes decrease and latencies increase until no response is visible at levels below threshold. VsEP thresholds for these two animals were scored at −13.5 and −10.5 db for Slc4a11^+/+ and Slc4a11^−/− mice, respectively.