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Growth assays of cells carrying Ark1p with WT or mutant AbpSH3 binding sites. The test strain was deleted for Prk1p and possessed an AbpSH3 binding site deletion in its chromosomal copy of Ark1p. Ark1p bearing the indicated AbpSH3 binding sites was expressed from a plasmid. Growth at 37 °C was dependent upon the interaction of the AbpSH3 with the plasmid-expressed Ark1p. Serial 10-fold dilutions of each culture were spotted on the plate. ArkA refers to the 17-mer ArkA.
