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. 2009 Jul 24;284(39):26951–26963. doi: 10.1074/jbc.M109.013706

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Biophysical and biochemical characterization of EcCSP and PpCSP by SDS-PAGE and immunoblot. A, Coomassie Blue-stained SDS-PAGE of 1 μg of purified recombinant EcCSP and PpCSP under nonreduced (NR) and reducing + alkylated (R/A) conditions. B, representative immunoblot of 0.5 μg of purified recombinant EcCSP-ML (lanes 1, 4, and 7), EcCSP-CL (lanes 2, 5, and 8), and PpCSP (lanes 3, 6, and 9) under nonreducing (lanes 1–3), reducing (lanes 4–6), and reducing + alkylated (lanes 7–9) conditions, and shown are results obtained using mAb 1G12.