. 2009 Jul 28;284(39):26999–27009. doi: 10.1074/jbc.M109.028589

TABLE 1.

Inhibition kinetics of wild-type and mutant PfDHODH

The error represents the standard error of the mean for n = 4–5. The fold change is shown in parentheses. The IC₅₀ values for A77 1726 against PfDHODH and hDHODH are 180 μm (19) and 0.3–1 μm (54–56), respectively. Brequinar binds hDHODH with an IC₅₀ of 0.006–0.01 μm (54–56).

Inhibitor enzyme	IC₅₀
Inhibitor enzyme	DSM1	DSM2	DSM74
		μm
PfDHODH	0.047^a	0.056^a	0.28^b
hDHODH	>100^a	>100^a	>100^b
PfDHODH-H185A	1.3 ± 0.17 (28)	1.3 ± 0.17 (23)	25 ± 9.0 (90)
PfDHODH-R265A	2.1 ± 0.70 (45)	2.4 ± 1.1 (43)	23 ± 6.7 (82)
PfDHODH-F227A	1.3 ± 0.086 (28)	4.4 ± 1.1 (79)	6.3 ± 1.1 (23)
PfDHODH-F188A	2.1 ± 1.4 (45)	0.21 ± 0.044 (4)	1.4 ± 0.37 (5)

^a Data taken from Ref. 18.

^b Data taken from Ref. 17.