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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Brain Res. 2009 Sep 11;1300:146–158. doi: 10.1016/j.brainres.2009.09.017

FIG. 8.

FIG. 8

Percent of cells showing positive staining for TUNEL assay in the cerebellar granule cell layer. Cryosections of cerebella of 58-day-old mice: untreated normal (nl unt), normal treated with minocycline (nl min), normal treated with indomethacin (nl ind), untreated affected (aff unt), affected treated with minocycline (aff min), affected treated with indomethacin (aff ind), affected treated with BMT (aff bmt), affected treated with both BMT and minocycline (aff bmt + min). Sections were stained with the ApopTag Plus Peroxidase In Situ Apoptosis Kit (Chemicon) and the total number of cells present in ten representative fields per sections were counted using high power magnification. The cells undergoing apoptosis are represented as percent of tunnel positive cells. Statistically significant decreases in number of apoptotic cells between treated mice and untreated mice were determined using the unpaired t test (*, P 0.01-0.05; **, P 0.001-0.01; ***, P < 0.001).