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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Cancer Res. 2009 Apr 7;69(8):3472–3481. doi: 10.1158/0008-5472.CAN-08-3886

Figure 1.

Figure 1

In vitro characterization of human GBM-derived cancer stem cells. A, typical appearance of “neurosphere” structures grown in the serum-free specific culture conditions (GBM8, left; BT74, right). Scale bars, 100 μm. B and C, immunocytochemical analysis of human GBM-SCs and their differentiated progeny. B, undifferentiated BT74 cells; C, differentiated BT74 cells. Left, staining for neural stem/progenitor marker nestin (Cy3, red); right, merged images of staining for astrocytic marker GFAP (Cy3, red) and neuronal marker βIII tubulin (FITC, green). A significant reduction in nestin expression and concomitant up-regulation of GFAP expression were observed after induction of differentiation. There are cells double positive for GFAP and βIII tubulin in the differentiated population (yellow, white arrows). Nuclei were visualized by staining with 4′,6-diamidino-2-phenylindole. Scale bars, 20 μm. D, flow cytometric analysis for neuronal stem cell marker CD133 on GBM-SC cultures. Dissociated neurospheres were stained either with isotype control (blue line) or anti-CD133/2 antibody (black line) conjugated with phycoerythrin before the analysis. The percentage of CD133-positive cells is indicated.