Fig. 1.

Selected factors affecting the expression of epidermal fatty acid-binding protein (E-FABP) in pheochromocytoma cells (PC12). (a) PC12 cells were treated with nerve growth factor (NGF, 50 ng/mL), insulin-like growth factor I (IGF I, 20 ng/mL), IGF II (20 ng/mL) or insulin (10 ng/mL) for 2 days. E-FABP mRNA was examined by Northern blot analysis. A representative blot of at least three independent experiments is shown. (b) PC12 cells were incubated with actinomycin D (ActD, 0.5 µg/mL), cycloheximide (CHX, 1 µg/mL), or forskolin (Fskl, 20 µM) in the presence or absence of NGF (50 ng/mL), for 24 h. E-FABP mRNA was evaluated by Northern blot analysis. A representative blot of at least three independent experiments is shown. (c) PC12 cells were treated with NGF for 3 days in the presence or absence of 30 µM U0126, mitogen-activated protein kinase kinase (MEK) inhibitor and the levels of E-FABP mRNA were analyzed by quantitative RT-PCR. Error bars represent the SEM of three independent experiments. Statistical significance between control and U0126-treated groups was determined by one-way anova. *p < 0.05.