Figure 3. Bone Marrow Monocytes Differentiate into Lung CD11b⁺ DCs during Respiratory Fungal Infection.

(A) Overview of experimental scheme. Recipient CD45.2⁺ mice received 8 × 10⁵ flow-sorted CD45.1⁺CD11c⁻CD19⁻Thy1.2⁻NK1.1⁻Ter119^−-GFP⁺ BM cells from CCR2 reporter mice prior to infection.

(B) Representative plots of single-cell lung suspensions from A. fumigatus-infected (left, right) or uninfected mice (middle) that received BM monocytes (middle, right) or no graft (left). The plots are gated on CD45.2⁺ cells (from 10⁶ total recorded lung cells), and the frequency of CD45.1⁺GFP⁺ cells and the number of events in each gate is indicated.

(C) The histograms depict CD11c and MHC class II expression by gated cells from infected (black line) or uninfected mice (filled gray histogram).