(A and B) CCR2 depleter mice received no toxin (control) or 10 ng/g body weight DT at day 0. At the indicated time points, mice were euthanized, and single-cell suspensions from the lungs (A) and spleens (B) were enumerated and analyzed by flow cytometry to determine toxin-induced cell depletion. For the top panel in (A), the baseline lung monocyte, CD103+ DC, CD11b+ DC, and lung macrophage counts (± SEM) were (in log10 units) 6.167 ± 5.645, 5.401 ± 4.557, 5.786 ± 4.825, and 5.512 ± 4.781, respectively. For the top panel in (B), the baseline spleen monocyte, CD8α+ DC, and CD11b+ DC counts (± SEM) were 5.746 ± 5.115, 4.978 ± 4.317, and 5.620 ± 4.916, respectively. One of three representative experiments is shown with 3–4 mice per group.