Skip to main content
. Author manuscript; available in PMC: 2009 Nov 30.
Published in final edited form as: J Immunol. 2009 Jun 26;183(2):1144–1154. doi: 10.4049/jimmunol.0900788

Figure 5. Macrophages and neutrophils migrate into the placental zone in response to CpG ODN in IL-10−/− mice.

Figure 5

(A–B) UMGC were harvested into single cell suspensions from control or CpG ODN treated IL-10−/− mice on gd9 and gd15. Histograms were gated from CD45+ cells for (A) F4/80+ or (B) Ly-6G+ cells. Histograms are representative of data from multiple experiments using 4 animals per condition. (A) IL-10−/− mice showed a significant increase in F4/80+ cells on gd9 and gd15, as compared to control ODN-treated mice, p<0.05. (B) Similarly, IL-10−/− mice showed a significant increase in Ly-6G+ cells on gd9 and gd15, as compared to control ODN-treated mice, p<0.05. (C–D) F4/80+ and GR1+ cells were evaluated by immunohistochemistry done on uteroplacental tissue collected on gd9 from CpG ODN treated IL-10−/− mice. The presence of (C) F4/80+ and (D) GR1+ cells migrating from the myometrium and into the developing placental zone on gd9 in response to CpG ODN was observed. (M) Demarcates the mesometrial triangle whereas (P) indicates the placental region.