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. 2009 Sep 16;1(4):e00016. doi: 10.1042/AN20090036

Figure 1. PC12-ND6 cells recapitulate the first four stages of neuronal differentiation.

Figure 1

(A) Morphological characteristics of PC12-ND6 cells. Untreated PC12 and PC12-ND6 (referred to as ND6) cells were labelled with the F-actin marker Alexa Fluor 488®–phalloidin (green), anti-β-III-tubulin antibody (red) and the nuclear marker TO-PRO®-3 (blue) and analysed by confocal microscopy. Scale bar, 10 μm. (B) Quantification of the distinct neuronal differentiation stages in cultured PC12-ND6 cells labelled with phalloidin 48 h after seeding. Results are means±S.D. for three independent experiments, with n = 200 cells per experiment.