Table 1. List of antibodies used for the immunofluorescence studies and immunoblot analyses.
Antibody | Type | Source (catalogue number) | Dilution | Application |
Zyxin | Mouse mAb | Abcam (ab50391) | 1:500 | IB, ICC |
Gelsolin | Mouse mAb | BD Bioscience (610412) | 1:200 | IB, ICC |
MAP-1A | Mouse mAb | Chemicon (MAB362) | 1:500 | ICC |
MAP-2 | Mouse mAb | Chemicon (MAB364) | 1:250 | ICC |
Total tau | Mouse mAb | Millipore (MAB361) | 1:500 | ICC |
Unphosphorylated tau | Mouse mAb | Chemicon (MAB3420) | 1:200 | ICC |
KIF5B | Mouse mAb | Abcam (ab28060) | 1:100 | IB, ICC |
Neurofilament M | Rabbit pAb | Chemicon (AB1987) | 1:200 | IB, ICC |
β-III-tubulin | Mouse mAb | Convance (MMS-435P) | 1:500 | ICC |
β-III-tubulin | Rabbit pAb | Abcam (ab18207) | 1:500 | ICC |
SOD2 | Rabbit pAb | Abcam (ab13534) | 1:500 | ICC |
COX III | Mouse mAb | MitoSciences (MS304) | 1:1000 | ICC, IB |
KIF5B | Mouse mAb | Abcam (ab28060) | 1:100 | ICC, IB |
GAPDH | Mouse mAb | Aplied Biosystems | 1:2000 | IB |
Actin | Mouse mAb | Chemicon (MAB15010) | 1:2000 | IB |
Alexa Fluor® 488-conjugated anti-rabbit IgG | Goat pAb | Invitrogen (A11034) | 1:1000 | ICC |
Alexa Fluor® 488-conjugated anti-mouse IgG | Goat pAb | Invitrogen (A11029) | 1:1000 | ICC |
Alexa Fluor® 488-conjugated anti-goat IgG | Donkey pAb | Invitrogen (A11055) | 1:1000 | ICC |
Alexa Fluor® 568-conjugated anti-rabbit IgG | Goat pAb | Invitrogen (A11036) | 1:1000 | ICC |
Alexa Fluor® 568-conjugated anti-mouse IgG | Goat pAb | Invitrogen(A11031) | 1:1000 | ICC |
Alexa Fluor® 647-conjugated anti-rabbit IgG | Goat pAb | Invitrogen (A21245) | 1:1000 | ICC |
Alexa Fluor® 647-conjugated anti-mouse IgG | Goat pAb | Invitrogen (A21236) | 1:1000 | ICC |