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. 2009 Sep 16;1(4):e00016. doi: 10.1042/AN20090036

Table 1. List of antibodies used for the immunofluorescence studies and immunoblot analyses.

IB, immunoblot analysis; ICC, immunocytochemistry; mAb, monoclonal antibody; pAb, polyclonal antibody.

Antibody Type Source (catalogue number) Dilution Application
Zyxin Mouse mAb Abcam (ab50391) 1:500 IB, ICC
Gelsolin Mouse mAb BD Bioscience (610412) 1:200 IB, ICC
MAP-1A Mouse mAb Chemicon (MAB362) 1:500 ICC
MAP-2 Mouse mAb Chemicon (MAB364) 1:250 ICC
Total tau Mouse mAb Millipore (MAB361) 1:500 ICC
Unphosphorylated tau Mouse mAb Chemicon (MAB3420) 1:200 ICC
KIF5B Mouse mAb Abcam (ab28060) 1:100 IB, ICC
Neurofilament M Rabbit pAb Chemicon (AB1987) 1:200 IB, ICC
β-III-tubulin Mouse mAb Convance (MMS-435P) 1:500 ICC
β-III-tubulin Rabbit pAb Abcam (ab18207) 1:500 ICC
SOD2 Rabbit pAb Abcam (ab13534) 1:500 ICC
COX III Mouse mAb MitoSciences (MS304) 1:1000 ICC, IB
KIF5B Mouse mAb Abcam (ab28060) 1:100 ICC, IB
GAPDH Mouse mAb Aplied Biosystems 1:2000 IB
Actin Mouse mAb Chemicon (MAB15010) 1:2000 IB
Alexa Fluor® 488-conjugated anti-rabbit IgG Goat pAb Invitrogen (A11034) 1:1000 ICC
Alexa Fluor® 488-conjugated anti-mouse IgG Goat pAb Invitrogen (A11029) 1:1000 ICC
Alexa Fluor® 488-conjugated anti-goat IgG Donkey pAb Invitrogen (A11055) 1:1000 ICC
Alexa Fluor® 568-conjugated anti-rabbit IgG Goat pAb Invitrogen (A11036) 1:1000 ICC
Alexa Fluor® 568-conjugated anti-mouse IgG Goat pAb Invitrogen(A11031) 1:1000 ICC
Alexa Fluor® 647-conjugated anti-rabbit IgG Goat pAb Invitrogen (A21245) 1:1000 ICC
Alexa Fluor® 647-conjugated anti-mouse IgG Goat pAb Invitrogen (A21236) 1:1000 ICC