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. 2009 Sep 23;1(4):e00017. doi: 10.1042/AN20090029

Figure 1. Generation of the JOE mouse.

Figure 1

(A) The construct was prepared using the 1.9 kb ‘classic' MBP promoter plasmid (pMG2; Gow et al., 1992). This promoter element contains a 1.9 kb sequence upstream of the classic MBP translation initiation site in exon 5B of the gene and two exon pieces of the β-globin gene to provide a splicing event. J37 cDNA was cloned into exon 3 of the β-globin gene. Since the 5′-portion of J37 cDNA contains the translation initiation site of classic MBPs, the initiator methionine was mutated to a leucine residue to ensure that no classic MBPs arose from this construct. (B) Western blot of samples from three JOE transgenic lines, ‘B', ‘D' and ‘A', at P24 probed with an antibody against classic MBP. J37 contains 133 amino acids of golli-specific peptide linked to classic MBP peptides 1–102 and 155–168. Our MBP antibody raised against amino acids 1−114 of bovine MBP recognizes J37 as well as classic MBPs.