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. 2009 May 5;158(4):1062–1073. doi: 10.1111/j.1476-5381.2009.00234.x

Figure 2.

Figure 2

GS-HNE stimulates acute peritonitis. To assess murine peritonitis, GS-HNE, free HNE or GS-DHN were injected intraperitoneally (each 10 µg) and the lavage was collected after 4 h. Total PMNs were determined by differential analysis using Wright–Giemsa staining (A) and confirmed by FACS analysis using PE-conjugated anti-Gr-1 antibodies (B; GS-HNE>>>GS-DHN>HNE). Results are presented as mean ± SEM and are representative of at least three independent experiments (n= 3–4) ***P < 0.001. GS-DHN, glutathionyl-1,4-dihydroxynonanol; GS-HNE, glutathionyl-4-hydroxy-trans-2-nonenal; PMN, polymorphonuclear leukocyte.