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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Struct Biol. 2009 Mar 6;168(1):95–106. doi: 10.1016/j.jsb.2009.02.013

Figure 3. Diffusion Through the Evanescent Wave.

Figure 3

(a) Schematic of diffusion close to a supported planar membrane. Fluorophores in the path of the evanescent wave are excited, and fluoresce. (b) Representative TIR-FCS data of diffusion, fit to an appropriate theoretical autocorrelation function. The data pertain to 10 nM Alexa448-labeled IgG in PBS with 1 mg mL−1 unlabeled IgG and 10 mg mL−1 ovalbumin diffusing close to a supported planar membrane. Reproduced with permission from Biophys. J. 2003. 85, 3294–3302.