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. Author manuscript; available in PMC: 2009 Nov 30.
Published in final edited form as: J Mol Biol. 2006 Mar 9;358(4):1060–1070. doi: 10.1016/j.jmb.2006.02.049

Figure 4.

Figure 4

Insertion of human FLJ20160 protein into membranes of E. coli or P. pastoris. Membrane fractions were separated from cytoplasm by centrifugation and samples containing 200 μg (a) or 50 μg (b) of protein were subjected to SDS/polyacrylamide gel (12%) electrophoresis. The gel was electroblotted to PVDF membrane and probed with HRP-conjugated antibody directed against His-tag. Molecular weight ladder is shown in kDa. (a) Membrane (lane 1) or cytoplasm (lane 2) fractions isolated from control untransformed E. coli XL1-Blue cells. Membrane (lane 3) or cytoplasm (lane 4) fractions isolated from E. coli XL1-Blue cells harboring pSP72 plasmid encoding human FLJ20160 protein. Purified LacY/His10 (0.2 μg; lane 5) is shown for comparison. (b) Membrane fractions isolated from different P. pastoris strains harboring pPICZ-A plasmid encoding human FLJ20160 protein after induction with methanol. KM71H (lane 1), X-33 (lane 2), and GS115 (lane 3).