TABLE 1.

Primer sequences of forked DNA substrates used in this study

Various forked DNA substrates were made by annealing the indicated primers. The primers were mixed at a 1:1 molar ratio and heated to 95 °C for 5 min, followed by slow cooling to room temperature. Unlabeled forked DNA (FkD-U) was used for the smPB experiment done with forked DNA and the various proteins (gp59, gp32, and gp41). Cy5-labeled forked DNA (FkD-Cy5) was used for the smPB co-localizing experiments done with proteins.

Name	Sequence
FkD-U	5′-Biotin-ACACAGACGTACTATCATGAGGTGAGGGAGGGTGGGTGGT-3′ (40-mer)
	5′-TGTGTCTGCATGATAGTACGGGTGAGGGAGGGTGGGTGGT-3′ (40-mer)
	5′-ACCCCCACCC-3′ (10-mer)
FkD-Cy5	5′-TATGAATCAGAGTGTAAGTTCCGAGTGATACAATGATAGTACGTCTGTGT-biotin-3′ (50-mer)
	5′-ACACAGACGTACTATCATGACGCATCAGACAACGTGCGTCAAAAATTACGTGCGGAAGGAGT-3′ (62-mer)
	5′-Cy5-ACTCCTTCCGCACGTAATTTTTGACGCACGTTGT-3′ (34-mer)