TABLE 2.

smPB data results

The K and σ values obtained from fitting the experimental data (Fig. 4) to Equation 4 are tabulated below. The K and σ values measure the magnitude of the association constant and relative strength of the binding events. The values are subgrouped and given as proteins with unlabeled forked DNA (FkD-U) in Nos. 1–4, with no DNA in Nos. 5–8, and with Cy5-labeled forked DNA (FkD-Cy5) in Nos. 9–12. Along with K and σ values and the residuals, a measure of the goodness of fit was obtained. The root mean square error obtained for all the fittings was less than ± 0.02. gp59*, Alexa 555-labeled gp59.

No.	System	K	σ
1	FkD-U + gp59*	0.90	0.86
2	FkD-U + gp59* + gp32	0.38	0.78
3	FkD-U + gp59* + gp41	0.64	0.97
4	FkD-U + gp59* + gp32 +gp41	0.68	1.2
5	gp59*	0.43	0.06
6	gp59* + gp32	0.27	0.03
7	gp59* + gp41	0.40	0.07
8	gp59* + gp32 + gp41	0.43	0.06
9	FkD-Cy5 + gp59*	0.71	0.73
10	FkD-Cy5 + gp59* + gp32	0.32	0.39
11	FkD-Cy5 + gp59* + gp41	0.93	0.87