FIGURE 1.

Components of the cADPR/CD38 signaling pathway in PC12 cells. A, expression of CD38 and RyR2 mRNA in PC12 cells were determined by RT-PCR. B, protein expression of CD38 and RyR2 in GFP-shRNA or CD38-shRNA-infected PC12 cells were determined by immunoblot analysis using either anti-CD38 or anti-Ryr antibody. C, Ca²⁺ increases in intact PC12 cells induced by a cell-permeant cADPR agonist, cIDPRE (200 μm), in the absence or presence of an antagonist of cADPR, 8-Br-cADPR (200 μm).