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. 2009 Aug 10;284(43):29365–29375. doi: 10.1074/jbc.M109.007971

FIGURE 3.

FIGURE 3.

ROCK-mediated JNK activation is required for PMA-induced apoptosis. A, LNCaP cells were treated with SP600125 (20 μm), added 1 h before PMA (100 nm, 1 h) or vehicle (ethanol) treatment. The incidence of apoptosis was determined 24 h later. B and C, LNCaP cells were treated with Y-27632 (10 μm) (B), cytochalasin B (2 μm), or blebbistatin (50 μm) (C), added 1 h before PMA treatment. Cells were then treated with 100 nm PMA for the indicated times (B) or for 30 min (C), and phospho-JNK or total JNK levels were determined by Western blot. The -fold increase in phospho-JNK levels is shown below the corresponding Western blots. D, LNCaP cells were treated with cytochalasin B (2 μm) or blebbistatin (50 μm), added 1 h before PMA or vehicle (ethanol) treatment. Cells were treated with PMA (30–100 nm) or vehicle for 1 h. The incidence of apoptosis was determined 24 h later. Results are presented as mean ± S.D. (n = 3). Two additional experiments gave similar results.