FIGURE 5.

Expression of BEST1 in HT₂₉ colonic epithelial cells. A, RT-PCR analysis (with (+) and without (−) reverse transcriptase) of the four human isoforms of bestrophin (BEST1–4) in HT₂₉ human colonic epithelial cells. B, summary of ATP-induced whole cell Cl⁻ conductances in HT₂₉ cells. nS, nanosiemens; TEA, tetraethylammonium. C, activation of whole cell currents in HT₂₉ cells by ATP (100 μm) under control (con) conditions and after incubation of the cells with BEST1 siRNA. D, Western blot analysis of BEST1 expression in HT₂₉ cells grown under control conditions after incubation with siRNA (RNAi) or after incubation with scrambled (scrbled) RNA. E, summary of the whole cell conductances activated in HT₂₉ cells grown under control conditions after incubation with siRNA or after incubation with scrambled RNA. The asterisks indicate significant difference compared with the control (paired Student's t test). The number of experiments is indicated in parentheses.