FIGURE 6.

POSH stimulates dynamin (Dyn)-dependent endocytosis of ROMK1. A, a whole cell potassium currents measured with the perforated whole cell patch recording in HEK293T cells transfected with GFP-ROMK1(R1), R1+POSH, R1+wtDyn, R1+wtDyn+POSH, R1+DynK44A, and R1+DynK44A+POSH (n = 4). The voltage protocol is the same as shown in Fig. 4 (from −100 to 60 mV). B, the potassium currents at −100 mV measured in different experiments are summarized in a bar graph. An asterisk indicates significant different (p < 0.05) in comparison with the control (ROMK1 alone). C, a Western blot shows the expression of corresponding proteins in all groups.