FIGURE 1.

Autophagy in MPSI and MPSVII liver is reduced by a high fat, simple sugar diet. MPSI (A) and MPSVII (B) LC3I and LC3II levels in liver were determined by Western blotting. Mice were fed the 22% fat diet (22%, A), the 42% fat diet (42%, A), the 13% fat diet (13%, B), or the Modified 42% fat diet (M42%, B). The dietary-induced change in LC3II levels in the MPSI (C) and MPSVII (D) Western were determined by densitometry and normalized to β-actin. Data shown are the average of 3 separate mice/diet ± 1 S.D. *, p < 0.05.